WebThermus thermophilus is a Gram-negative bacterium used in a range of biotechnological applications, including as a model organism for genetic manipulation, structural … WebDerived from pMK18, allowing overexpression of ParAB m in T. thermophilus: This study: Strains: HB27: T. thermophilus wild type: DSM7039: Δbgl: T. thermophilus HB27 derivative with bgl gene clean deleted (Angelov et al. 2013) ΔparAB m: T. thermophilus HB27 derivative with parAB m deleted: This study: ΔparB c::kat-parB c _sgfp
Cre/ lox -based multiple markerless gene disruption in the
WebMar 21, 1994 · In Thermus thermophilus HB27, the crtB gene encoding phytoene synthase was found to exist on the large plasmid, pTT27. One of the carotenoid under-producing mutants, Crt31, carried a derivative of pTT27 (pTT27') in which deletion and inversion were observed near the crtB gene. T. thermophilus HB8 also contained a large plasmid which … WebComparison of multiple T. thermophilus genomes indicated that the genes for xylose metabolism were present on a plasmid in only one strain. Inspection of metagenomic dataset for adapted communities that contain T. thermophilus indicated that the plasmid is present in the T. thermophilus populations but may be lost upon isolation. KW - Consortium. robe constantine
Thermus thermophilus as a Source of Thermostable Lipolytic Enzymes …
WebThe pCT3FK shuttle fosmid carries additional selection and mobilization elements for the Thermus host, in particular a thermostable kanamycin resistance cassette and constitutive slp promoter (de Grado et al., 1999) and a sequence of the pyrE locus for chromosomal integration of the vector in T. thermophilus HB27 via WebIsolation of a phage infectious to HB27. T. thermophilus In order to isolate a novel myovirus, we screened hot water of Atagawa hot spring with HB27 as an indicator strain. Several plaques were found and one of them was cloned by two serial passages of phage dilutions. The newly isolated phage, designated WebRecent work has shown that DNA can be transferred by conjugation from T. thermophilus strain HB8 to a closely related strain, HB27. Strain HB8 harbors a region of its chromosomal DNA similar in sequence to the F plasmid of E. coli which allows conjugal transfer of chromosomal genes after it integrates into the chromosome of the donor cell. robe cool femme