Primescript rt reagent kit with gdna
WebPrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (20 μl RT x 100 rxns) RR064A: One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) 6210A : PrimeScript™ II 1st Strand cDNA Synthesis Kit (50xns) Real Time PCR: RR420A : SYBR® Premix Ex Taq™ (Tli RNaseH Plus), 5mL RR820A : TB ... WebPrimeScript Reverse Transcriptase synthesizes first-strand cDNA efficiently from standard oder complex (e.g., GC-rich) RNA templates. ... Media construction kits; One-step RT-PCR; PrimeScript cDNA synthesis kits. PrimeScript IV first-strand cDNA synthesis mix; PrimeScript two-step kit;
Primescript rt reagent kit with gdna
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WebRNA quantity and quality were determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Massachusetts, USA). cDNA was synthesized with the PrimeScript RT reagent kit with gDNA Eraser (TAKARA, Dalian, China) according to the manufacturer's instructions. Briefly, 1 µg total RNA was used to erase gDNA at 42°C for 2 … WebMar 1, 2024 · Takara. RR037B (A × 4) PrimeScript ™ RT reagent Kit (Perfect Real Time) 200 Rxns × 4. ¥7,456. ¥4,474. *红字为促销价格,促销时间: 2024年3月1日-2024年4月30日. …
WebPrimescript Rt Reagent Kit With Gdnaeraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 265 PubMed citations. ZERO BIAS - …
WebUSD $380.00. PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. It uses PrimeScript Reverse Transcriptase, which … WebNov 28, 2024 · Total RNA was extracted from PCa cell lines by TRIzol reagents from Invitrogen/Life Technologies (Carlsbad, CA, USA) and reverse transcribed to complementary DNA (cDNA) by PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa ... of each well by Spectrophotometer (EPOCH3, BioTek, USA). The KIT standard sample established the ...
WebOne microgram of total RNA in a final volume of 20 μL was used for reverse transcription with PrimeScript RT reagent Kit with gDNA Eraser (Takara) and T100™ Thermal cycler (Bio-Rad). qRT–PCR was performed on the CFX96 PCR system (Bio-Rad) by using TB Green Premix Ex Taq II (Takara) to detect the expression of the relevant genes following the …
WebApr 12, 2024 · Reverse transcription was conducted to generate complementary DNA (cDNA) using the Prime Script RT reagent kit with gDNA Eraser according to the manufacturer’s protocol. The mRNA expression of IL-6, and ABCA1 was determined by quantitative real-time PCR using SYBR premix Ex Tap TM (TLiRNSEHPLUS). half inched itWebFeb 1, 2024 · For cDNA synthesis, 2.5 ng of total RNA was reverse transcribed (RT) into cDNA using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time, TaKaRa, Japan) according to the manufacturer’s instructions. The real-time monitoring of PCR amplification reaction was performed on a Real-Time PCR Detection System ... bunbury glass and glazingWebMar 1, 2024 · The RNA integrity was analyzed by electrophoresis on 1% agarose gels. Synthesis of first-strand cDNA from each total RNA sample (300 ng) was performed using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara, Dalian, China) according to the manufacturer's protocol. 2.3. Primer design and PCR efficiency bunbury gatesWeb21 hours ago · After detection by 1% gel electrophoresis, the total RNA was first treated to remove the genomic DNA and then transferred into the first strand of cDNA using the PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Dalian, China) according to the manufacturer’s instructions. The first strand of synthesized cDNA was used as a template. half inch easy outWebTaKaRa primescript rt reagent kit with gdna eraser Primescript Rt Reagent Kit With Gdna Eraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based … half inch embroidery fontsWebMar 1, 2024 · PrimeScript RT reagent Kit with gDNA Eraser是可以除去基因组DNA进行Real Time RT-PCR反应的专用反转录试剂。. Kit中使用了具有较强DNA分解活性的gDNA … half inch drive six point indestro socketsWebStructured Review. TaKaRa. Primescript Rt Reagent Kit With Gdna Eraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … bunbury glass