Lanthascreen
WebbThe LanthaScreen™ kinase activity assay toolbox has been designed with four key goals in mind: To provide users the ability to rapidly apply TR-FRET technology ; To develop …
Lanthascreen
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WebbInvitrogen • LanthaScreen™ Estrogen Related Receptor alpha TR-FRET Coactivator Assay Page 3 of 13 3. INTRODUCTION Invitrogen’s LanthaScreen™ Estrogen Related Receptor alpha TR-FRET Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential ERR alpha ligands as inverse agonists … WebbLanthaScreen® Eu Kinase Binding Assay Validation Packet Version No.: 2 Page 1 of 18 Optimization of a LanthaScreen® Eu Kinase Binding Assay for LCK Have a question? …
Webbthese events in a high-throughput screening (HTS) environment. LanthaScreen™ Ubiquitin and SUMO assay reagents were specifically designed for use in HTS applications. This … WebbLanthaScreen® Eu-anti-Tag Antibody Emission 615 nm (10 nm bandpass) Dichroic Mirror Instrument dependent Delay Time 100 µs Integration Time 200 µs For additional …
WebbLanthaScreen® Eu Kinase Binding Assay Validation Packet Version No.: 1 Page 1 of 17 Optimization of a LanthaScreen® Eu Kinase Binding Assay for HCK Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 Select option 3, ext. 40266 Email: [email protected] WebbLanthaScreen™ Eu Kinase Binding Assay Screening Protocol and Assay Conditions Revised 07-Jun-2016 Page 2 of 9 Assay Theory The principle of the LanthaScreen Eu Kinase Binding Assay is shown in Figure 1. Binding of an Alexa Fluor™ conjugate or “tracer” to a kinase is detected by addition of a Eu-labeled anti-tag antibody.
WebbTime-resolved fluorescence (TRF) reduces background by using a lanthanide fluorophore, such as europium or terbium, that emits long-lived fluorescence. This long-lived fluorescence lasts for milliseconds, so excitation of the fluorophore by a pulsed light source (e.g., a flash lamp), followed by a delay and then signal measurement (‘counting ...
WebbShop LanthaScreen™ Cellular Assay Lysis Buffer at Fishersci.se LanthaScreen™ Cellular Assay Lysis Buffer 100mL LanthaScreen™ Cellular Assay Lysis Buffer … happy birthday in portuguese imageWebbObtain sensitive detection of kinase inhibitors, including allosterics. Interrogate active and non-activated kinases. Track slow-binding inhibitors in real time. Compare inhibitor on … chair turns into tableWebbinstrument is properly set-up and capable of detecting TR-FREt signals in LanthaScreen® Assays. We designed the LanthaScreen® Tb technical note to use components and reagents that are generally used in most LanthaScreen® Assays. Please note that the LanthaScreen® GFP-tagged physiological substrates and Nuclear Receptor … happy birthday in pythonWebbThis LanthaScreen® Eu Microplate Reader Test provides a method to verify the ability of your fluorescent plate reader to detect a change in time-resolved fluorescence energy … chair tufted swivelWebbLanthaScreen™ Tb anti-GFP Antibody is a terbium-labeled antibody recognizing green fluorescent protein (GFP). It can be used to determine GFP expression levels in any GFP expressing cell line with greater sensitivity than detecting the fluorescence intensity of the GFP protein alone. happy birthday in portuguese songWebbShop LanthaScreen™ Cellular Assay Lysis Buffer at Fishersci.se LanthaScreen™ Cellular Assay Lysis Buffer 100mL LanthaScreen™ Cellular Assay Lysis Buffer Fisher Scientific English chairty application login #WebbOptimization of a LanthaScreen™ Eu Kinase Binding Assay for ACVR1. 2. Dilute the “Master Dilution” series 33.3-fold into Kinase Buffer A. Remove 5 µL of each concentration of diluted compound, transfer to another 96-well plate, add 162 µL of Kinase Buffer A and mix (Figure 6, Step 2). 3. chair tufted recliner small