How to make kanamycin stock solution
WebChloramphenicol - 10 mL (1,000 X Stock) 34 mg/ml chloramphenicol in ethanol. Reagents needed: 0.34 g. chloramphenicol. 10 ml. 100% ethanol. Directions: 1) Dissolve 0.34 g of chloramphenicol into 10 ml 100% ethanol. 2) Filter through a 0.22 µm filter to sterilize. 3) Aliquot and store at -20°C. Web27 okt. 2008 · Weigh 800mg of kanamycin into a small weigh boat. Add 80ml of milliQ to a 250ml bottle. Add the kanamycin to the milliQ; Mix/vortex so all the kanamycin goes …
How to make kanamycin stock solution
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Web29 mei 2016 · For BG11 solid medium, add 15 g of agar and 700 ml of water to one flask. To the second flask, add 3 g of Na 2 S 2 O 3, 226 ml of water, 10 ml of 100x BG11, 1 ml of trace elements and 1 ml of iron stock.Autoclave both solutions. After these solutions have cooled to room temperature, combine them and add 1 ml of phosphate stock, 1 ml of Na … WebCreate a stock solution of your antibiotic. Unless otherwise indicated, the antibiotic powder can be dissolved in dH 2 0. Addgene recommends making 1000X stock solutions and …
http://www.its.caltech.edu/~bjorker/Protocols/Pouring%20LB_Agar_Antibiotic%20Plates%20092016.pdf Web15 nov. 2013 · Most of the stock solutions are prepared as a 1000X concentration, so you will add 0.5 mL of stock antibiotic per 500 mL of solution. (Aliquots of Carbenicilin and Kanamycin are located in the -20C fridge by Bay D) Typical antibiotic FINAL concentrations; Carbenicllin 100 µg/mL Ampicillin 100 µg/mL Kanamycin 50 µg/mL 5.
WebHow to Prepare a Sterile Antibiotic Stock Solution Using an EZ Pak™ Gold Biotechnology, Inc. 5.95K subscribers Subscribe 74 8.7K views 3 years ago Lab Tutorials Thanks for watching! Gold... Web18 rijen · * Generally, prepare 30-50 mL of solution in a 50 mL conical tube. Then, filter …
WebPreparation of 10 ml of 50 mg/ml ampicillin stock solution in water. PROCEDURE. Step 1: Dissolve 500 mg of Ampicillin Sodium in 9 ml of Milli Q water. Weigh out and transfer 500 …
Web1. Weigh 0.5 g of Kanamycin (Kanamycin (Monosulfate), GoldBio Catalog # K-120 [CAS 25389-94-0, mw. = 582.6]). 2. Add 10 ml of sterile H 2 O. Dissolve completely. 3. … phone number for brightspeedWebKanamycin: Prepare stock solution, filter sterilize using a 0.2 µm syringe filter (B-D # 309602 - Fisher Catalog # 09-719C or equivalent), and store at 2-8°C (for 3 weeks maximum) or-20°C or lower for long-term storage. Add appropriate amount to sterile culture medium (after cooling to ~ 50oC) how do you pronounce shinoWeb1. Take Ampicillin powder from the -20°C and weigh out 1 g on the analytical balance. 2. Place the weighed amount into a 15 mL conical vial that has 10 mL of water in it. 3. Shake vigorously until all is dissolved. 4. Obtain a 12 mL sterile syringe and .2 um sterile filter. 5. Obtain a second 10 mL centrifuge tube. 6. Clean and sterilize the hood. how do you pronounce shingrixWebQ. chloramphenicol stock solution chloramphenicol stock solution 만들 때, 20ml ethanol에 500mg chloramphenicol을 넣어서 25mg/ml로 만들려고 하는데요,,, 공업용 ethanol을 써도 되나요?: A. 공업용이면 순도가 낮아서 문제가 되지 않을까요? 어차피 chloramphenicol 만들어서 세포나 동물에 투여할텐데 말이죠 phone number for brightlineWeb12 apr. 2024 · Pre-warm the solution at 37 °C using an Eppendorf ThermoMixer. 7. In the same microcentrifuge tube, add 6 μL of the 2OG stock solution, 1 μL of the peptide stock solution, and 1 μL of the ascorbate solution. Mix using a vortex mixer. Immediately before the reaction, add 1 μL of the 1 mM Fe(II) stock solution, and mix using a vortex mixer. phone number for brighthouse financialWebTetracycline must always be wrapped in foil and stored in the dar k whether it is in stock solution, in broth, or in agar. !" Stock solutions in water must be filter sterilized through a 0.22-µm filter. Antibiotics dissolved in ethanol need not be sterilized. !" Do not use expired antibiotics to make stock solutions, as they may be labile and ... phone number for brinks customer serviceWebAdd 40 μL of 100 mM Thermo Scientific IPTG Solution, ready-to-use (Cat #R1171) 5. Spread evenly on the plate with a sterile spatula. For batch use, add the following directly per 1 mL of the liquid LB agar (kept at about 50 °C): 6. 1 μL of X-Gal Solution (20 mg/mL), ready-to-use. 7. 1 μL of 100 mM IPTG Solution, ready-to-use. phone number for brightstar bank in florida