2024 Hieff clone plus multi one step cloning kit

Hieff clone plus multi one step cloning kit

Author: liqh

August undefined, 2024

WebClonExpress II One Step Cloning Kit . 非连接酶依赖型单片段快速克隆试剂盒. 快速克隆；高通量克隆；无缝克隆；DNA定点突变；双链DNA与载体组装. · 简单、快速、高效， … Webcloned into the HindIII/EcoRI sites of shuttle vector pWHU2581 using Hieff Clone® Plus Multi One Step Cloning Kit (Yeasen, China). The gene (aprI, aprJ, aprZ) knockout …

Cloning Bioline Meridian Bioscience

Web9 de dez. de 2024 · HR donor plasmids were constructed using the Hieff Clone Plus Multi One Step Cloning Kit (YEASEN, China). The G622A HR donor was constructed by ligating four fragments (a left arm, ... WebGostaríamos de lhe mostrar uma descrição aqui, mas o site que está a visitar não nos permite. termine ljpa düsseldorf

Rapamycin targets STAT3 and impacts c-Myc to suppress tumor …

WebBasic Steps of Cloning. There are many approaches available for cloning DNA, most of which follow this basic workflow: Isolation of target DNA - typically referred to as an … WebBGC0001040: rapamycin biosynthetic gene cluster from Streptomyces rapamycinicus NRRL 5491. WebGostaríamos de lhe mostrar uma descrição aqui, mas o site que está a visitar não nos permite. termine m3 2024

Frontiers Juvenile Hormone Membrane Signaling Enhances its ...

Hieff Clone™ Zero TOPO-TA Cloning Kit-Yeasen

WebUniversal one step cloning kit, a one step cloning kit, is developed by seamless cloning technology based on homologous recombinase. Compared with the traditional enzyme … roanoke rapids nc hondaWebHieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒，精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反应所需缓冲液，并添加了独特的重组增强因子，可显著提高重 … termine kts

"Web27 de abr. de 2024 · PCR of the coding sequence of Met or Gce from the pClone007-Met or pClone007-Gce vector was performed, and then we used a Hieff Clone Plus Multi One Step Cloning Kit (10912ES10, Yeasen) for assembly. Finally, we obtained the pUAST-Met-4 × FLAG-Avi-P2A-BirA ( UAS-Met-BirA ) or pUAST-Gce-3 × FLAG-Avi-P2A-BirA ( UAS … " - Hieff clone plus multi one step cloning kit

Hieff clone plus multi one step cloning kit

1 2 a Deep-intronic and frameshift DSG1 variants 1 2 a ... - Springer

WebClone High: Created by Bill Lawrence, Phil Lord, Christopher Miller. With Will Forte, Phil Lord, Michael McDonald, Christa Miller. The greatest minds of the world have been … WebThe Hieff Clone™ Universal One Step Cloning Kit should be stored at -25℃~ -15°C for 1 year. Figures. Figure 1 Multi-segment linkage: performance comparison with competitive …

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Web12 de ago. de 2024 · The full-length cDNA of OsEBF1 (LOC_Os06g40360) was amplified with KOD-plus DNA polymerase (Toyobo, Tokyo, Japan) using the primers EBF1OE-attB-F and EBF1OE-attB-R, and cloned into the p1301-35SNos vector using a Hieff-Clone™ Plus Multi One Step Cloning Kit (Yeasen, Beijing, China). WebThe Hieff Clone™ Universal One Step Cloning Kit ensures efficient single- and multi-fragment cloning. A-B: Recombinant transformation plates. C: Electropherogram of …

WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, … WebGenBuilder™ Cloning Kit: GenBuilder™ Plus Cloning Kit: 1: Unique need fulfilled by the kit: Multi-gene cloning for up to 6 fragments: Multi gene cloning for up to 12 fragments: 2: Cost per reaction: $7.90: $9.80: 3: Number of fragments that can be cloned into a linearized vector: 6: 12: 4: Cloning efficiency (insert was cloned into the ...

http://yeasen.com/assets/upload/file/20240817130924833.pdf Web24 de abr. de 2024 · In summary, the multiple genes transformation increased the SF content in broccoli more than single gene introductions. The aim of this research was to improve sulforaphane content by introducing FMOGS–OX2, ... (Hieff Clone Plus Multi One Step Cloning Kit, Yeasen, shanghai, China).

WebFigure 5. Single fragment cloning of 2290 bp was performed with the Hieff Clone™ Plus One Step Cloning Kit. A: line 1 is the linear vector, and line 2 is the target DNA …

Web产品描述. Hieff Clone TM Plus Multi One Step Cloning Kit 是一款简便、快速、高效的 DNA 定向克隆产品，该试剂盒可以将 PCR 产物定向克隆至任何载体的任何位点，可高效克隆 50 bp-10 kb 片段。将载体线性化，并在插入片段正、反向 PCR 引物 5’ 端引入 15-25 bp 的线性化载体末端同源序列，使得插入片段 PCR 产物 ... termine mmgWeb20 de mai. de 2024 · The purified PCR product was then cloned into the pCAMBIA1300-UBI-RBCS vector using the Hieff Clone® Plus One Step Cloning Kit. The D3 knockout vector was constructed using the CRISPR/Cas9 method. Based on the genomic sequence of D3 gene, two specific CRISPR target sites were designed in the protein-encodable … termine megalomaneWeb14 de fev. de 2024 · 图8：Hieff Clone TM Plus Multi One Step Cloning Kit与不同品牌的同类产品相比，显示出更高的性价比。 FAQ. Q1 ： Hieff Clone TM 克隆的原理是什 … roanoke prideWebBest practices. With focuses on the research and development and manufacturing of tool enzyme raw materials, antigen and antibodies, Yeasen supplies 40+ types of enzymes, … roanoke pontoon boatWeb27 de fev. de 2024 · Hieff Clone TM Plus Multi One Step Cloning Kit was purchased from YEASEN Biotechnology Co., ... using Hieff Clone Plus Multi One Step Cloning Kit, … termine kv saarlandWeb11 de nov. de 2024 · The In-Fusion cloning based on in vitro homologous recombination was performed to generate all the constructs using an In-Fusion HD Cloning Kit (Clontech) and Hieff Clone Plus Multi One Step ... termine m2 2024WebElisa Kit / ... Hieff Clone ® 多片段一 ... 10912ES10. 10 T. Hieff Mut TM 定点突变试剂盒. 11003ES10. 10 T . HB211123. 推荐商品 (10911ES) Hieff Clone ® Plus One Step … termine markus krebs 2023