Web2.4 U/ml dispase II (Boehringer Mannheim Corp.) from -20˚C frozen aliquot 2.5 mM CaCl2 Use immediately. F-10-based primary myoblast growth medium 400 ml Ham’s F-10 nutrient mixture (GIBCO BRL) 100 ml fetal calf serum (HyClone Laboratories, Inc.) (20% final) WebSep 17, 2024 · Dispase: 1.25 U/mL: 5 mL (from 2.5 U/mL stock solution prepared according to manufacturer's instructions) CaCl 2: 2 mM: 20 μL (from 1 M stock solution CaCl 2 prepared according to manufacturer's instructions) b. Store the freshly prepared muscle digestion buffer at 4°C until needed.
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WebSkin tissue from 3 to 5 days neonatal mice was floated on 1 U/ml dispase (Corning, 354235) in Hank's balanced salt solution (HBSS) (Gibco, 14170) for 16 hr at 4°C with the dermis side down. WebSTZ1 & STZ2, 0.22μ filtered STEMxyme® AOF Collagenase/Neutral Protease (Dispase®) ... This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of HBSS or equivalent yields a solution of 300 units/ml of collagenase, Code: CLSPA. Suitable for cell isolation and culture applications. town of greenburgh ny tax collector
Posterior vitreous detachment with dispase - PubMed
Web诺为生物是eBioscience, MiltenyiBiotec,STEMCELL, SunJinLab, Lucigen,Novobiotec,LGC Biosearch,iBiochips授权代理商,是干细胞,免疫学研究的专业化服务平台,引进smart … WebDispase II (neutral protease) is an amino-endo peptidase that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. This sequence is found with high … Web5. Add 2 mL of pre-warmed Dispase (5 U/mL) and 200 μL of DNase I Solution (1 mg/mL). Pipette the sample for 1 minute with a 1 mL pipettor to further dissociate cell clumps. The sample should now be cloudy, but not stringy. If still stringy, add an additional 100 µL of DNase I Solution and pipette as above. 6. town of greenburgh ny school tax