WebNov 16, 2024 · The wavelength you use for excitation of the donor and the wavelength you are detecting in your emission channel from acceptor should be well selected. For the … WebThe FRET efficiency is then calculated as the following ratio: Fa / (Fd + Fa), where Fa is the acceptor emission and Fd is your donor emission. Assuming that BSA is your donor and the drug your ...

Cy3 and Cy5 dyes attached to oligonucleotide terminus stabilize …

WebJun 18, 2011 · 他们将Cy3 Cy5分别作为供体和受体标记到 a2原肌球蛋白 (aTm,是aa 同二聚体) 上,当用光激发 Cy3 时,可以 观察到FRET现象的发生;当蛋白质变性,成为两条 多肽链 … Webdistance. For FRET to take place the emission spectrum of the donor molecule has to overlap with the absorption spectrum of the acceptor molecule.2,3 The spectral overlap for each FRET pair is shown in Figures S 2AC (A: FAM and Cy5, B: FAM and Cy3, C: Cy3 and Cy5). FRET - between FAM and Cy5 is unlikely because the spectral overlap is ... tabc and texas food handlers certification

荧光染料之IF、FITC、TRITC、青色素 - 知乎

Webオリゴヌクレオチドの5′末端を下図のようにCy3およびCy5で標識します。 ドナーとなるCy3から発せられる光によりCy5が励起され、発光します。 ヌクレアーゼを加えるとオリゴヌクレオチドが切断され、2つの蛍光物質が離れるため、FRETが起こらなくなります。 WebAlexa Fluor 680 dye. Invitrogen Alexa Fluor 680 dye is a bright, near-IR fluorescent dye with excitation ideally suited for the 633 nm laser line. For stable signal generation in imaging … WebCy3 and Cy5 were co-localized by the software and time traces of fluorescence intensities for both fluorophores were plotted (Fig. 3B). Under continuous laser illumination, both Cy3 and Cy5 were photobleached - Cy5 faded faster than Cy3 most of the times. The traces of Cy3 and Cy5 were inversely correlated with each other for a typical FRET ... tabc application investment