Commonly misidentified cell lines
Web460 rows · This database lists misidentified cell lines as well as cell lines that have … WebJun 8, 2016 · The risk is very real – there are now over 400 misidentified cell lines registered in the Database of cross-contaminated or misidentified cell lines. ... A recent …
Commonly misidentified cell lines
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WebContaminated cell lines have been extensively used in research without knowledge of their true character. For example, most if not all research on the endothelium ECV-304 or the megakaryocyte DAMI cell lines has in reality been conducted on bladder carcinoma and erythroleukemia cells, respectively.
WebProblematic cell line: Misidentified. Originally thought to be a hepatocellular carcinoma cell line but shown to be from an hepatoblastoma (PubMed=19751877). Part of: Cancer Cell Line Encyclopedia (CCLE) project. Part of: ENCODE project common cell types; tier 1. Part of: JFCR45 cancer cell line panel. Part of: MD Anderson Cell Lines Project. WebJan 24, 2024 · The pervasiveness of cross-contaminated and misidentified cell lines is a decades-long issue; in 1967, cell lines thought to be derived from various tissues were shown to be HeLa cells, a human cervical adenocarcinoma cell line. 1 However, studies involving these misidentified cell lines continued to feature in hundreds of citations …
WebAuthentication of a cell line is the sum of the process by which a line’s identity is verified and shown to be free of contamination from other cell lines and microbes. Download this technical bulletin to learn how to achieve reliable and reproducible experimental data. Download the technical bulletin Featured Resources Human Cell STR Profiling (1) WebJan 16, 2024 · The commonest is HeLa (140 entries), followed by T-24 (21 entries) and M14 (18 entries). The ICLAC Register of Misidentified Cell Lines was originally developed by Amanda Capes-Davis and Ian Freshney, and published in 2010. It is now curated by the … This worksheet for human cell line authentication can help scientists to …
WebMay 31, 2024 · Abstract. Continuous cell lines are widely used, but can result in invalid, irreproducible research data. Cell line misidentification is a common problem that can …
WebContaminated cell lines have been extensively used in research without knowledge of their true ... billy walters las vegasWebSep 13, 2024 · In this review, we highlight the common pitfalls when working with antibodies, common practices for validating antibodies, and levels of commercial antibody validation for seven vendors. ... First step (and simplest): check your cell line(s) against the list of known cross-contaminated or misidentified cell lines available on the NCBI … cynthia key obituaryWebApr 17, 2024 · Indeed, worldwide, between 14 and 46% of the most commonly used cell lines are believed to be incorrectly designated [7 ... From January 2010 to January 2016, using the misidentified cell line HBL-100, we were able to find only two articles that referred to this cell line in the title or abstract. Both were submitted in 2009, which was … cynthia keyworthWebMake sure your cells have not been misidentified and reclassified. Cell lines are among the most used in vitro models representing normal and disease tissue in basic research … cynthia keyserWebHEK293T cells (ATCC® CRL‐3216™, RRID: CVCL_0063) (the cell line is not listed as a commonly misidentified cell line by the International Cell Line Authentication Committee) were cultured… No further authentication was performed in the laboratory. A maximum of 6 cell passages was used. All data are presented as mean ± SE. cynthia key rural hall ncWebOct 31, 2024 · Cancer cell lines are widely used in biomedical research; they provide excellent model systems to study mechanisms associated with cancer development, … billy walters net worth 2021http://iclac.org/wp-content/uploads/Cross-Contaminations-v8_0.pdf cynthia key tillo facebook