2024 Bcdat medium

Bcdat medium

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WebSep 8, 2024 · The cellophane and cultures were then transferred onto BCDAT agar medium containing various concentrations of arginine and cultured for an additional 7 days. The pH of the BCDAT medium was 6.5, but was modified to 8.5 with KOH to prepare an increased-pH control for BCDAT medium supplemented with 3.0 mM arginine. … WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m -2 s -1 ): dark …

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WebFeb 8, 2024 · BCDAT medium was additionally supplemented with 1 mM ammonium tartrate 50. Plants were grown at 24 o C with a 16 h: 8 h, light (300mmol m-2 s-1): dark cycle. To induce sporophytes, tissues were grown for 3-6 months on peat plugs at 16°C with an 8 h: 16 h, light (300mmol m-2 s-1): dark cycle. Sporangia were sterilized in 20% … WebSep 12, 2024 · They were then moved to BCDAT plates supplemented with 50 μg/ml hygromycin by transfer of the cellophane discs. After 10-14 days, cellophane disks were transferred to BCDAT medium without antibiotic. After an additional 10-14 days, growing colonies were transferred back to BCDAT plates supplemented with hygromycin for a … redistributable for visual studio 2012

Phytophthora infestans RXLR effector AVR1 disturbs the growth …

WebDec 1, 2024 · Gametophore colonies grown on BCDAT medium for 40 days were used for the analysis of phenotypic characterization. The Branching patterns and the number of each module classes were measured according to the branching forms as previously described [23]. The lengths of internode were measured using ImageJ. 2.7. WebSep 20, 2024 · The moss leaf cells were cultured in BCDAT medium. Liberalities, the information entropy of transcriptome were measured in each culture time. Figure 2: Bar charts of leaf cells transcriptome during reprogramming. The occupation rate of genes in a transcriptome was plotted in a bar chart. Heights of boxes in a bar chart indicate the … WebJul 22, 2024 · Survivors were transferred to plates containing BCDAT medium, and then the plates were vertically oriented in darkness for phenotypic screening (Fig. 1a ). Several mutants whose protonemata grew... redistributable bgp

Metabolic Control of Gametophore Shoot Formation

WebFeb 12, 2024 · BCDAT medium : Add 10 mL of stock B, 10 mL of stock C, 10 mL of stock D, 10 mL of 500 mM ammonium tartrate, 1 mL of alternative TES, and 20 mL of 50 mM CaCl 2 to 900 mL of water, and fill to 1000 mL with water. For solid medium, add 8 g of agar per 1000 mL of medium. 3. BCDATG medium : BCDAT medium supplemented with 5 g/L … WebAug 17, 2024 · The gametophores were then covered with solid BCDAT medium with 10 µg ml −1 PI without zeocin, and observation was resumed. Bright-field and PI fluorescence images of two or three leaves of ... redistributable filesWebDec 1, 2024 · All plant materials were cultured on BCDAT medium with 1 mM CaCl 2 at 25℃ under a 16 h/8 h (light/dark) photoperiod with the light intensity of 60−80 μmol … richard allen long

"Web, 0.17 mM KI) agar medium supplemented with 1mM CaCl 2 and 5mM ammonium tartrate (BCDAT medium), or as lawns of protonemal ﬁlaments by subculture of homoge-nized tissue on BCDAT agar medium overlaid with cellophane for the isolation of protoplasts. Bacterial strains and constructs Plasmid Tnk23 (Lucas etal., 1995) is a derivative of the … " - Bcdat medium

Bcdat medium

NO GAMETOPHORES 2 Is a Novel Regulator of the 2D to 3D …

WebApr 19, 2024 · P. patensprotonemal tissue was subcultured routinely at 7-day intervals on cellophane disks (AA packaging) overlaying Petri dishes (90 mm in diameter) that … WebJan 26, 2024 · After regeneration on PRMB medium containing 3 µM PABA, four-day-old transformed protoplasts were selected on BCDAT medium containing 3 µM PABA, 100 mg/l gentamicin, and 20 mg/l G418 for one week. Transformants were picked to BCDAT+PABA medium, then 10 days later a small clump of each was transferred to …

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Webtoplasts per plate) containing BCDAT medium with mannitol and incubated in the light (15Wm 2) for 6d. Antibiotic-resistant plants were selected by transfer of the cellophane … WebJan 28, 2024 · GFP-mTalin transformants for cdka-dko deficiency were selected on BCDAT medium containing hygromycin (30 mg/liter) or zeocin (100 mg/liter) and further confirmed by polarotropism defects under polarized light. To observe cp-actin, a confocal laser scanning microscope was used (Zeiss LSM 510). The objective lens was a Plan …

WebJun 1, 2016 · (E–H) Plants grown for one month on BCDAT medium to promote filamentous growth. (E, F) WT plant grown without auxin or with 12.5 μM NAA, respectively. (G) iaa2 … WebJun 1, 2024 · Finally, half of the gametophores of S0, S1 and R1 were used for total RNA extraction, and half of R1 gametophores were transferred to BCDAT medium for continued growth. Arabidopsis thaliana ( A. thaliana ) seeds, ecotype Col-0 were washed with 75% Ethanol for 5 min, 5% sodium hypochlorite for 10 min, then washed with water for 10 …

WebNov 18, 2024 · BCDAT medium is composed of 1 mM MgSO 4, 1.84 mM KH 2 PO 4 (pH 6.5), 10 mM KNO 3, 45 μM FeSO 4, trace element solution (0.22 μM CuSO 4, 10 μM H 3 … WebOct 23, 2024 · A microdevice with protonema cells was submerged in BCDAT medium to prevent liquid evaporation from the channels; the dish was sealed with parafilm and left for 3–4 d at 25 °C under continuous ...

WebApr 8, 2024 · patens protonemal tissue was grown on BCDAT medium with and without β-estradiol for 2 days and total RNA was isolated using Trizol and a RNeasy mini kit …

WebDec 7, 2015 · Transformants were confirmed after three rounds of selection on BCDAT medium supplemented with G418 medium (30 μg·mL −1) alternatively for 15 days each … redistributable epic gamesWebJun 1, 2016 · The body plan of the early-diverged moss Physcomitrella patens (P. patens) is relatively simple. The vegetative gametophyte is composed of only a few cell types and developmental stages. Auxin was … redistributable latestWebFeb 6, 2024 · Protonemata were dispersed in liquid BCDAT medium containing bleomycin or MMS and treated for 1 h. After treatment and washing recovered protonemata were inoculated as eight explants per quadrant of a Petri dish with drug-free BCDAT agar without cellophane overlay and left to grow. redistributable for mysqlWebMay 25, 2024 · BCDAT medium: BCD medium supplemented with 5 mM diammonium (+)-tartrate. 3. BCDATG medium: BCDAT medium supplemented with 0.5% glucose. 2.4 Solutions and Media for Transformation 1. 8% Mannitol: Dissolve 8 g D-mannitol in 100 ml H 2 O and sterilize by autoclaving. Store at room temperature. 2. richard allen martinWebDec 12, 2024 · Three-week-old protonemata of WT, ko lines and complemented lines grown on BCDAT medium were observed using a light microscope. Phenotypes of protonemal cells were observed in WT, ko lines and complemented lines. (a) ppkp4-1, ppkp4-2 and ppkp4-1/2 knockout mutants showed dead cells in protonemata. richard allen legacyWebBCD medium. Do not include CaCl 2 if the medium is to be used to prepare protoplast regeneration media (PRMB, PRMT, or PRML). If using CaCl 2, add after autoclaving, … redistributable latest supportedWebSep 1, 2012 · A mixture of chloronemata and caulonemata was homogenized by vortexing for 1 minute using a six-well tube and ceramic balls (KURABO, Osaka, Japan), and vegetatively propagated on a BCDAT medium agar plate for four days. Then, the tissues were treated with or without phytohormones. redistributable for games